›› 2011, Vol. 23 ›› Issue (4): 279-283.doi: 10.3969/j.issn.1004-616x.2011.04.008

• 论著 • 上一篇    下一篇

Survivin调控相关基因在新疆哈萨克族食管癌中的表达

郭文佳, 张丽萍, 王洪江, 李 卉, 庞作良, 李惠武2，*   

  1. 1. 新疆医科大学附属肿瘤医院；2. 新疆医科大学基础医学院，新疆 乌鲁木齐 830011
  • 收稿日期:2011-03-10 修回日期:2011-05-19 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 郭文佳

Expressions of Survivin related genes in Xinjiang Kazak's esophageal cancer

GUO Wen-jia1，ZHANG Li-ping，WANG Hong-jiang，LI Hui，PANG Zuo-liang，LI Hui-wu，*   

  1. 1. Department of Thoracic Surgery, Affiliated Cancer Hospital of Xinjiang Medical University; 2. Pre-clinical Medicine College of Xinjiang Medical University, Urumqi 830011, Xinjiang, China
  • Received:2011-03-10 Revised:2011-05-19 Online:2011-07-30 Published:2011-07-30
  • Contact: GUO Wen-jia

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摘要： 目的： 探讨与凋亡相关因子Survivin调控相关的基因c-IAP2、Bcl-2、STAT3、cyclinD1、PTEN、VEGF在新疆哈萨克族食管癌中的作用。方法：从50例新疆哈萨克族食管癌标本中筛选出Survivin mRNA差异表达 (癌组织中的表达量高于远端无癌组织)的32例癌组织及远端无癌组织标本，采用RT-PCR方法检测c-IAP2、Bcl-2、STAT3、cyclinD1、PTEN、VEGF mRNA在标本中的表达；并分析这些基因的表达与临床病理因素间的关系。结果：在哈萨克族食管癌中，c-IAP2、Bcl-2、STAT3、cyclinD1、PTEN、VEGF mRNA的表达阳性率依次为93.75%，78.13%、68.75%、65.63%、87.5%和84.38%；半定量光密度扫描结果显示，c-IAP2、STAT3、PTEN、VEGF mRNA在癌组织中的表达均显著高于无癌组织 (P均＜ 0.05)；Survivn mRNA的阳性表达率与c-IAP2、STAT3、PTEN、VEGF mRNA的表达呈正相关；在食管癌组织中cyclinD1 mRNA的表达与c-IAP2、Bcl-2、PTEN mRNA的表达具有相关性 (P ＜0.05)， PTEN的表达与c-IAP2、STAT3、cyclinD1、VEGF mRNA的表达具有相关性 (P ＜0.05)；cyclinD1 mRNA的表达与哈萨克族食管癌组织的分化程度相关，Bcl-2、STAT3 mRNA的表达与食管癌淋巴结转移相关，VEGF mRNA的表达则与食管癌患者性别、癌组织分化程度相关 (P＜0.05)。结论：Survivin调控相关的基因c-IAP2、Bcl-2、STAT3、cyclinD1、PTEN、VEGF在新疆哈萨克族食管癌发生发展过程中可能发挥着重要的作用。

关键词: 新疆哈萨克族食管癌, Survivin, 细胞凋亡, 细胞周期, 血管新生

Abstract: OBJECTIVE: To investigate the expression of c-IAP2，Bcl-2，STAT3，cyclinD1，PTEN， VEGF associated with apoptosis -ralated gene Survivin in Kazak’s esophageal carcinoma tissues and its adjacent non-carcinoma tissues and to find relationships between Survivin and the above six genes. METHODS：RT-PCR was used to detect the expressions of these six genes in carcinoma tissues and its adjacent non-carcinoma tissues in 32 Kazaks esophageal cases，Western-bolt performed to evaluate the levels of proteins. RESULTS：In Xinjiang Kazak's esophageal cancer，The expression rates of these six genes in 32 cases were over 50.0％，with c-IAP2 at 93.75%，cyclinD1 at 87.5%，VEGF at 84.38%. The expression levels of c-IAP2 STAT3 PTEN and VEGF were significantly increased in carcinoma compared to the adjacent non-carcinoma tissues(P<0.05). There were differences in the expressions of c-IAP2，STAT3，PTEN and VEGF mRNA between carcinoma and its adjacent non-carcinoma tissues (P<0.05). PTEN and VEGF showed similar expression status in the level of transcription and translation. There were correlations between Survivin and c-IAP2，STAT3，PTEN and VEGF expressions in carcinoma tissues (P<0.05). We found that the expression of cyclinD1 correlated with c-IAP2，Bcl-2 and PTEN mRNA；and the expression of PTEN correlated with c-IAP2， TAT3， cyclinD1 and VEGF mRNA (P<0.05). Expression of cyclinD1 correlated with differentiated degree and stage；the expression of Bcl-2 and STAT3 correlated with lymph node metastasis whilst the expression of VEGF correlated with sex，lymph node metastasis (P<0.05). CONCLUSION：Survivin played an important role in promoting carcinogenesis and development of esophageal cancer in Kazak's，through regulating apoptosis，cell cycle and angiogenesis. These six genes could be identified as molecular markers for early diagnosis，and prognosis prediction in Xinjiang Kazak's esophageal cancer.

Key words: Xinjiang Kazak's esophageal cancer, Survivin, apoptosis, cell cycle, angiogenesis

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